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A Brief History Of The Development Of Colloidal Gold

wallpapers News 2020-12-22
Gold sol, also known as colloidal gold, is a stable, uniform, and single-dispersed suspension of gold particles suspended in a liquid after the gold salt is reduced to gold. The gold sol particles are composed of a gold atom and a double ion layer surrounding it.
A brief history
The use of colloidal gold as a marker in immunohistochemistry began in 1971. Faulk et al. used electron microscopy immunogold staining (IGS) to observe Salmonella, after which they combined colloidal gold with a variety of proteins. In 1974, Romano et al. labelled colloidal gold on the secondary antibody (horse anti-human IgG) and established an indirect immunocolloidal gold staining method.
In 1978, geoghega discovered the application of colloidal gold markers at the level of light microscope. This application of colloidal gold in immunochemistry is also called immunogold. After that, many scholars further confirmed that colloidal gold can adsorb proteins stably and quickly, and the biological activity of the protein has not changed significantly. It can be used as a probe to accurately locate biological macromolecules such as polysaccharides, proteins, polypeptides, antigens, hormones, nucleic acids, etc. on the cell surface and in the cells. It can also be used for daily immunodiagnosis and immunohistochemical localization. The application of drug testing and other aspects has received extensive attention.
At present, immunogold staining (IGS) at the electron microscope level, immunogold-silver staining at the light microscope level (IGSS), and dot immunogold staining at the naked eye level are increasingly becoming powerful tools for scientific research and clinical diagnosis. Colloidal properties The size of colloidal gold particles is mostly 1-100nm, and the tiny gold particles are stably, uniformly, and suspended in the liquid in a single dispersion state to become a colloidal gold solution. In the past 10 years, colloidal gold labeling has developed into an important immunolabeling technique. Colloidal gold immunoassays have been developed in many fields such as drug detection and biomedicine, and have received more and more attention in related research fields.